Maeda Lab:Protocols

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Protocols <Molecular Biology> qPCR setup and analysis with the Stratagene Mx3000P DNA-free RNA isolation Arabidopsis gDNA isolation for Genotyping Miniprep, Alkaline Lysis qPCR analysis - Agilent Stratagene Mx3000 RNA isolation from plant tissues E. coli competent cell preparation Cloning using In-Fusion system (Clontech) Site-directed mutagenesis using In-Fusion system Sequence analysis NCBI Blast Fold & Function Assignment System (FFAS) Multiple Alignment BoxShade Select "RTF_new" and "ALN" Reverse Complement Convert Sequence Format Translate Protein Targeting Prediction (ChloroP, Predotar,TargetP, PSORT) <Biochemistry> Protein Modeling using Swiss-Model and PyMOL <Analytical Chemistry> Tocopherol analysis - HPLC Amino acid analysis - HPLC and GCMS <Plant Growth> Seed Sterilization - Arabidopsis, Beets Useful links Research ApE plasmid Editor Arabidopsis growth ARBC Sorghum genome blast Clint Chapple Lab at Purdue University Plasmid Isolation - Mini Prep video Others Waste Pickup Request UW Madison Chemical Safety Dept (waste disposal etc) Botany Department IT resources and introduction Lab Notebook Guidelines from WARF Reference managers: Mendeley and Zotero How to place an Article and Book request at UW Library How to merge PDF files Media Wiki Editing Consult the User's Guide for information on using the wiki software. Configuration settings list MediaWiki FAQ MediaWiki release mailing list Noble Foundation Medicago insertional mutant collection