Difference between revisions of "Maeda Lab:Protocols"

Protocols

<Molecular Biology>

• qPCR analysis - Agilent Stratagene Mx3000
• RNA isolation from plant tissues
• E. coli competent cell preparation
• Cloning using In-Fusion system (Clontech)
• Site-directed mutagenesis using In-Fusion system

• Sequence analysis
  • NCBI Blast
  • Fold & Function Assignment System (FFAS)
  • Multiple Alignment
  • BoxShade
    • Select "RTF_new" and "ALN"
  • Reverse Complement
  • Convert Sequence Format
  • Translate
  • ChloroP
  • PSORT

<Biochemistry>

• Protein Modeling using Swiss-Model and PyMOL

<Analytical Chemistry>

• Tocopherol analysis - HPLC
• Amino acid analysis - HPLC and GCMS

<Plant Growth>

• Seed Sterilization - Arabidopsis, Beets

Useful links

• ApE plasmid Editor
• Arabidopsis growth ARBC
• Sorghum genome blast
• Clint Chapple Lab at Purdue University
• Botany Department IT resources and introduction
• Lab Notebook Guidelines from WARF
• Reference managers: Mendeley and Zotero
• Plasmid Isolation - Mini Prep video

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