Difference between revisions of "Maeda Lab:Protocols"

Protocols

<Molecular Biology>

• qPCR setup and analysis with the Stratagene Mx3000P
• DNA-free RNA isolation
• Arabidopsis gDNA isolation for Genotyping
• Miniprep, Alkaline Lysis
• qPCR analysis - Agilent Stratagene Mx3000
• RNA isolation from plant tissues
• E. coli competent cell preparation
• Cloning using In-Fusion system (Clontech)
• Site-directed mutagenesis using In-Fusion system

• Sequence analysis
  • NCBI Blast
  • Fold & Function Assignment System (FFAS)
  • Multiple Alignment
  • BoxShade
    • Select "RTF_new" and "ALN"
  • Reverse Complement
  • Convert Sequence Format
  • Translate
  • Protein Targeting Prediction (ChloroP, Predotar,TargetP, PSORT)

<Biochemistry>

• Protein Modeling using Swiss-Model and PyMOL

<Analytical Chemistry>

• Tocopherol analysis - HPLC
• Amino acid analysis - HPLC and GCMS

<Plant Growth>

• Seed Sterilization - Arabidopsis, Beets

Useful links

• Research
  • ApE plasmid Editor
  • Arabidopsis growth ARBC
  • Sorghum genome blast
  • Clint Chapple Lab at Purdue University
  • Plasmid Isolation - Mini Prep video
• Others
  • Waste Pickup Request
  • UW Madison Chemical Safety Dept (waste disposal etc)
  • Botany Department IT resources and introduction
  • Lab Notebook Guidelines from WARF
  • Reference managers: Mendeley and Zotero
  • How to place an Article and Book request at UW Library
  • How to merge PDF files

Media Wiki Editing

Consult the User's Guide for information on using the wiki software.

• Configuration settings list
• MediaWiki FAQ
• MediaWiki release mailing list
• Noble Foundation Medicago insertional mutant collection